But the exact same thing would happen if our sample started with 183,001 cells.
So let s get started already.
The worried nurse hands you a vial of 10 mLs of Frank s blood.
I need a hint :Assume you are starting with 1,000,000,000 cells in one mL of solution another hint :After first dilution you are left with 10,000,000 cells in one mL of solution dilution factor 0 another hint :Dilution factor is the same each time another.So our absolutely unavoidable error is plus or minus 500/182,000 - about.27.Blaaah, still too much.Even if you have perfect technique in mixing and pipetting, you cannot determine the exact number of bacteria present in your sample.If we count a,000 diluted plate, we should find 183 CFUs remember, we have magically flawless technique.
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Luckily, the kingdom measured in metric only.
Make sure you understand the following concepts - go back to the applets if you need to: dilution factor stated as a fraction or decimal overall dilution factor product of individual dilution factors choosing the plate to count 30 to 300 CFUs scaling up from.
Given the following series of dilutions, what is the total dilution factor.Overall dilution factor.1.1.01.0001 Multiply by the inverse I think I have the answer: 48 pb full client 2013 1/0.0001 480,000 CFUs per mL I did a series of dilutions as follows: 1 mL added to 9 mLs water 1 mL added to 99 mLs water.So, for example, you might estimate that in one square urban mile there is on average one barber shop just barbers, not hairdressers with 2 barbers.What are the pitfalls.It doesn t really matter how much stock you start with, the important thing is to take out the right fraction of it a tenth, a hundredth and then add enough water to get back to the initial volume.The prince tried the triple-diluted soup.Anyone can eat a soup that has 90 peas per liter.Also, notice in this problem I didn t tell you how much the small bag weighed, only that it contained 1/11 of the amount of a large bag.